Melanocortin signalling in fat cells

• Type: Article in National Scientific Journal
• Date: 2009

Authors

Rodrigues, Adriana (Author) FCNAUP; Almeida, Henrique (Author) FCNAUP; Gouveia, Alexandra (Author) FCNAUP

Journal

Title: Revista Portuguesa de Endocrinologia, Diabetes e Metabolismo

Vol. 4 No. 2

Pages: 152-152

ISSN: 1646-3439

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Resumo (PT): [resumo]

Abstract (EN): Melanocortin peptides and their receptors have long been known to affect the central control of food intake and body weight, thus playing a critical role in the development of obesity [1]. The melanocortin 5 receptor (MC5R) has been directly linked with lipid metabolism in peripheral tissues: MC5R deletion in mouse generates defects in sebaceous gland secretion [1] and MC5R stimulation increases fatty acid oxidation in muscle cells [2]. In this study, we tackled the role of MC5R in lipolysis and adipocyte function using the well established model of differentiated mouse 3T3-L1 adipocytes. Cell-signalling based experiments were performed after adipocyte treatment with alpha-melanocyte-stimulating hormone (alpha-MSH) and compared with the MC5R specific activation previously described in HEK293 [3] and HeLa cells stably transfected with MC5R-GFP. Adipocyte response to alpha-MSH resulted in a loss of cytoplasmatic lipid content and release of glycerol into the cell medium by an ERK1/2 dependent mechanism. Moreover, downstream of ERK1/2, alpha-MSH induces p90RSK and CREB phosphorylation and c-Fos expression. The correlation between alpha-MSH action in 3T3-L1 adipocytes and the specific signalling pathways underlying MC5R activation, strongly suggests that lipolysis is mediated by this receptor. [1] Cone RD (2006) Endocr Rev 27(7):736:49 [2] An et al (2007) J Biol Chem 282(5):2862-70 [3] Rodrigues et al (2009) Mol Cell Endocrinol 303:74-81

Language: English

Type (Professor's evaluation): Scientific