The effect of high glucose on SERT, the human plasmalemmal serotonin transporter

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Gonçalves P (Author) FMUP; Araújo JR (Author) FMUP; Martel, F (Author) FMUP

Journal

Title: Nutritional Neuroscience

Vol. 11

Pages: 244-250

ISSN: 1028-415X

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-003-TPS

DOI: 10.1179/147683008x344156

Abstract (EN): The aim of this work was to investigate the effect of short- and long-term high-glucose exposure on the plasmalemmal serotonin transporter (SERT)-mediated uptake of [(3)H]-serotonin (5-HT) by Caco-2 cells. Short-term exposure of Caco-2 cells to high apical glucose levels (30 mM for 2 h or 40 mM for 1 h) decreased the uptake of [(3)H]-5-HT by 20-30%. On the other hand, long-term (21-24 weeks) exposure of the cells to high (25 mM) glucose caused a 30% increase in the uptake of [(3)H]-5-HT. Under these conditions, the affinity of the transporter for 5-HT and noradrenaline was not significantly changed, and the inhibitory potencies of fluoxetine and desipramine were also unchanged. In conclusion, high-glucose levels modulate SERT activity. A short- term exposure of the cells to a high concentration of glucose decreases the activity of the transporter, whereas a longer exposure of the cells to a high concentration of glucose increases the activity of SERT, without interfering with its affinity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7