Synthesis, structural characterization, cytotoxic properties and DNA binding of a dinuclear copper(II) complex

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ferreira, BJML (Author) Other; Brandao, P (Author) Other; Meireles, M (Author) Other; Martel, F (Author) FMUP; Correia Branco, A (Author) Other; Diana M Fernandes (Author) FCUP; Santos, TM (Author) Other; Felix, V (Author) Other

Journal

Title: Journal of Inorganic Biochemistry

Vol. 161

Pages: 9-17

ISSN: 0162-0134

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 29 Citations

Other information

Authenticus ID: P-00K-MRX

DOI: 10.1016/j.jinorgbio.2016.04.026

Abstract (EN): In this study a novel dinuclear copper(II) complex with adenine and phenanthroline has been synthesized and its structure determined by single crystal X-ray diffraction. In the dinuclear complex [Cu-2(mu-adenine)(2)(phen)(2)(H2O)2](NO3)(4)center dot 0.5H(2)O (phen = 1,10-phenanthroline) (1) the two Cu(II) centres exhibit a distorted square pyramidal coordination geometry linked by two nitrogen donors from adenine bridges leading to a Cu-Cu distance of 3.242(3) angstrom. Intramolecular and intermolecular pi center dot center dot center dot pi interactions as well as an H-bonding network were observed. The antitumor capacity of the complex has been tested in vitro against human cancer cell lines, cervical carcinoma (HeLa) and colorectal adenocarcinoma (Caco-2), by metabolic tests, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide as reagent. The complex 1 has remarkable low IC50 values of 0.87 +/- 0.06 mu M (HeLa) and 0.44 +/- 0.06 mu M (Caco-2), when compared with values for cisplatin against the same cell lines. The interaction of complex 1 with calf thymus DNA (CT DNA) was further investigated by absorption and fluorescence spectroscopic methods. A binding constant of 5.09 x 10(5) M-1 was obtained from UV-vis absorption studies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9