Modulation of glucose uptake in a human choriocarcinoma cell line (BeWo) by dietary bioactive compounds and drugs of abuse

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Araújo JR (Author) FMUP; Gonçalves P (Author) FMUP; Martel, F (Author) FMUP

Journal

Title: Journal of Biochemistry

Vol. 144

Pages: 177-186

ISSN: 0021-924X

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 37 Citations

Scopus - 41 Citations

Other information

Authenticus ID: P-003-XBK

DOI: 10.1093/jb/mvn054

Abstract (EN): The aim of this work was to investigate the putative modulation of glucose uptake in trophoblast cells by several dietary compounds and by drugs of abuse. For this, the acute (26 min) and chronic (48 h) effect of these substances on the apical uptake of (3)H-2-deoxy-D-glucose ((3)H-DG) by a human choriocarcinoma cell line (BeWo) was determined. (3)H-DG apical uptake by BeWo cells was time dependent, displayed saturable kinetics (V(max) = 1210 +/- 29 nmol mg protein(-1) 6 min(-1) and K(m) = 13.4 +/- 0.5 mM) and was insulin-insensitive and cytochalasin B-sensitive (by up to 60%). Acutely, acetaldehyde (30-100 mM), resveratrol, xanthohumol, epigallocatechin-3-gallate (100 mu M), chrysin and quercetin (10-100 mu M) decreased (3)H-DG apical uptake, whereas rutin, catechin (10-100 mu M), epicatechin (100 mu M) and ethanol (10 mM) increased it. Quercetin and xanthohumol seem to be non-competitive inhibitors of (3)H-DG apical uptake, whereas both epigallocatechin-3-gallate and acetaldehyde decreased both the K(m) and V(max) values. Chronically, rutin and myricetin increased the apical uptake of (3)H-DG both isolated (0.1-1 mu M) and in combination (both at 1 mu M), whereas theophylline (0.1-1 mu M) and amphetamine, 3,4-methylenedioxymethamphetamine (0.25-1 mu M) and Delta(9)-tetrahydrocannabinol (1 nM) decreased it. In conclusion, (3)H-DG apical uptake by BeWo cells is differentially modulated by different compounds present in drinks and by drugs of abuse.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10