Uptake of 1-methyl-4-phenylpyridinium (MPP+) by the JAR human placental choriocarcinoma cell line: Comparison with 5-hydroxytryptamine

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Martel, F (Author) FMUP; Elisa Keating (Author) FMUP

Journal

Title: Placenta

Vol. 24

Pages: 361-369

ISSN: 0143-4004

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 19 Citations

Other information

Authenticus ID: P-000-H9G

DOI: 10.1053/plac.2002.0917

Abstract (EN): The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP+) in the JAR human choriocarcinoma cell line. As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of H-3-MPP+ and H-3-serotonin (H-3-5HT) was made. Specific uptake of H-3-MPP+ (0.2 mum) was temperature-, Na+- and potential-dependent. 5HT and MPP+ reduced H-3-MPP+ specific uptake (for 5HT, its IC50 was found to be 4 mum). The SERT inhibitors desipramine and fluoxetine also inhibited H-3-MPP+ specific uptake (with IC50S of 189 and 0.92 mum, respectively). The inhibitors of the extraneuronal monoamine transporter (EMT) and of the organic cation transporter type 2 (OCT2), corticosterone and decynium22, had no effect on H-3-MPP+ specific uptake, but cyanine863 concentration-dependently reduced it (with an IC50 of 23 mum). Specific uptake of H-3-5HT (0.2 mum) by JAR cells was temperature-, Na+- and potential-dependent. 5HT, MPP+, desipramine and fluoxetine concentration-dependently inhibited H-3-5HT specific uptake (with IC50S of 1-9 muM ,50 mum, 0. 17 mum and 0.046 mum, respectively). Corticosterone showed no effect, but decynium22 and cyanine863 significantly reduced 3 H-5HT specific uptake. For cyanine863, its IC50 was found to be 11 mum. In conclusion, the results suggest that: (1) uptake of H-3-5HT by JAR cells occurs exclusively through SERT; (2) uptake of H-3-MPP+ by JAR cells involves SERT and also another transporter; (3) neither EMT nor OCT2 are functionally present in JAR cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9