HPV16 is sufficient to induce squamous cell carcinoma specifically in the tongue base in transgenic mice

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Verónica F. Mestre (Author) Other; Beatriz Medeiros-Fonseca (Author) Other; Diogo Estêvão (Author) Other; Fátima Casaca (Author) Other; Sandra Martins (Author) FCUP; Ana Félix (Author) Other; Fernanda Silva (Author) Other; Bruno Colaço (Author) Other; Fernanda Seixas (Author) Other; Margarida M. S. M. Bastos (Author) FEUP; Carlos Lopes (Author) Other; Rui Medeiros (Author) ICBAS; Paula A. Oliveira (Author) Other; Rui M. Gil da Costa (Author) Other

Journal

Title: Journal of Pathology

Vol. 251

Pages: 4-11

ISSN: 0022-3417

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 30 Citations

Other information

Authenticus ID: P-00R-VMD

DOI: 10.1002/path.5387

Abstract (EN): Head and neck squamous cell carcinomas (HNSCCs) associated with human papillomavirus (HPV) occur specifically in the tonsils and the tongue base, but the reasons for this specificity remain unknown. We studied the distribution of oral and pharyngeal lesions in HPV16-transgenic mice where the expression of all the HPV16 early genes is targeted to keratinising squamous epithelia by the cytokeratin 14 (Krt14) gene promoter. At 30 weeks of age, 100% of mice developed low- and high-grade intraepithelial dysplasia at multiple sites. Twenty per cent of animals developed invasive cancers that remarkably were restricted to the tongue base, in association with the circumvallate papilla. The lesions maintained expression of CK14 (KRT14) and the HPV16 E6 and E7 oncogenes, and displayed deregulated cell proliferation and up-regulation of p16(INK4A). Malignant lesions were poorly differentiated and destroyed the tongue musculature. We hypothesised that the tongue base area might contain a transformation zone similar to those observed in the cervix and anus, explaining why HPV-positive cancers target that area specifically. Immunohistochemistry for two transformation zone markers, CK7 (KRT7) and p63 (TP63), revealed a squamocolumnar junction in the terminal duct of von Ebner's gland, composed of CK7(+) luminal cells and p63(+) basal cells. Dysplastic and invasive lesions retained diffuse p63 expression but only scattered positivity for CK7. Site-specific HPV-induced carcinogenesis in the tongue base may be explained by the presence of a transformation zone in the circumvallate papilla. This mouse model reproduces key morphological and molecular features of HPV-positive HNSCC, providing a unique in vivo tool for basic and translational research. (c) 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8