Immunophenotypic aberrations, DNA content, and cell cycle analysis of plasma cells in patients with myeloma and monoclonal gammopathies

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Lima, M (Author) Other; Dos Anjos Teixeira, M (Author) Other; Fonseca, S (Author) Other; Goncalves, C (Author) Other; Guerra, M (Author) Other; Luis Queiros, M (Author) Other; Santos, AH (Author) Other; Coutinho, A (Author) Other; Pinho, L (Author) Other; Marques, L (Author) Other; Cunha, M (Author) Other; Ribeiro, P (Author) Other; Xavier, L (Author) Other; Vieira, H (Author) Other; Pinto, P (Author) Other; Justica, B (Author) Other

Journal

Title: Blood Cells, Molecules, and Diseases

Vol. 26

Pages: 634-645

ISSN: 1079-9796

Publisher: Elsevier

Indexing

Scopus - 32 Citations

Other information

Authenticus ID: P-007-9AS

DOI: 10.1006/bcmd.2000.0342

Abstract (EN): We describe the immunophenotypic and gross DNA defects in 55 patients with myeloma and 50 patients with monoclonal gammopathy and review the literature on this subject (MedLine, 1994-2000). Our data confirmed previous reports indicating that in myeloma nearly all marrow plasma cells are abnormal (98.7 ± 8.1%). In monoclonal gammopathy the fraction of abnormal plasma cells was 35.0 ± 32.8%. In both myeloma and monoclonal gammopathy, the most frequent aberrant phenotypic features consisted of absence of expression of CD19, strong expression of CD56, and decreased intensity of expression of CD38; aberrant expression of CD10, CD20, CD22, or CD28 was observed in less than one-third of myeloma cases. The vast majority of cases had two or more phenotypic aberrations. In the DNA studies, 7% of myeloma cases were biclonal and 93% of cases were monoclonal. In those studies with only one plasma cell mitotic cycle, 37% had normal DNA content and 63% were aneuploid (hyperploid, 61%; hypoploid, 2%). The mean percentages of plasma cells in S- and G2M phases were 4.9 ± 8.5 and 4.4 ± 6.9%, respectively. Thirty-eight percent of cases had more than 3% of plasma cells in S phase. In monoclonal gammopathy, the DNA index of abnormal plasma cells ranged from 0.89 to 1.30 and the percentage of diploid (31%) and aneuploid (69%) cases was not different from the results found in myeloma. The differences in percentage of abnormal plasma cells in S- (7.4 ± 8.6%) and G2M-phases (2.4 ± 1.7%) in patients with monoclonal gammopathy were not statistically significant. © 2000 Academic Press.

Language: English

Type (Professor's evaluation): Scientific