THE GEL TEST - SOME PROBLEMS AND SOLUTIONS

• Type: Article in International Scientific Journal
• Date: 1992

Authors

DEFIGUEIREDO, M (Author) Other; Lima, M (Author) Other; MORAIS, S (Author) Other; Graca Porto (Author) ICBAS; JUSTICA, B (Author) Other

Journal

Title: Transfusion Medicine

Vol. 2

Pages: 115-118

ISSN: 0958-7578

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-001-PJS

Abstract (EN): The gel centrifugation test (GT) is a method of transfusion serology, based on the fact that, after centrifugation, unagglutinated red blood cells (RBC) pass easily through a gel, while agglutinated RBC do not. The introduction of the GT to our blood bank transfusion routine [strictly following the manufacturer's instructions (DiaMed ID Micro Typing System)] resulted in problems with the interpretation of the results. These were overcome after the introduction of modifications, which included; (1) the systematic use of 1% RBC suspensions; (2) the use of 50-mu-l of 1% RBC suspensions and 25-mu-l of serum in all tests; (3) the control of all negative indirect antiglobulin tests (IAT) and direct antiglobulin tests (DAT) by the addition of 50-mu-l of a 1% IgG coated RBC suspension followed by centrifugation; and (4) the systematic use of saline-suspended RBC for ABO typing in patients with positive DAT.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4