Profiling DNA Methylation Based on Next-Generation Sequencing Approaches: New Insights and Clinical Applications

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Barros Silva, D (Author) Other; Joana Marques, CJ (Author) FMUP; henrique, rmf (Author) ICBAS; Carmen Jeronimo (Author) ICBAS

Journal

Title: Genes

Vol. 9

Final page: 429

ISSN: 2073-4425

Publisher: MDPI

Indexing

ISI Web of Knowledge - 85 Citations

Scopus - 109 Citations

Other information

Authenticus ID: P-00P-K06

DOI: 10.3390/genes9090429

Abstract (EN): DNA methylation is an epigenetic modification that plays a pivotal role in regulating gene expression and, consequently, influences a wide variety of biological processes and diseases. The advances in next-generation sequencing technologies allow for genome-wide profiling of methyl marks both at a single-nucleotide and at a single-cell resolution. These profiling approaches vary in many aspects, such as DNA input, resolution, coverage, and bioinformatics analysis. Thus, the selection of the most feasible method according with the project's purpose requires in-depth knowledge of those techniques. Currently, high-throughput sequencing techniques are intensively used in epigenomics profiling, which ultimately aims to find novel biomarkers for detection, diagnosis prognosis, and prediction of response to therapy, as well as to discover new targets for personalized treatments. Here, we present, in brief, a portrayal of next-generation sequencing methodologies' evolution for profiling DNA methylation, highlighting its potential for translational medicine and presenting significant findings in several diseases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12