A Systematic AQbD Approach for Optimization of the Most Influential Experimental Parameters on Analysis of Fish Spoilage-Related Volatile Amines

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Freitas, J (Author) Other; Silva, P (Author) Other; vaz-pires, p (Author) ICBAS; Camara, JS (Author) Other

Journal

Title: Foods

Vol. 9

Final page: 1321

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00S-R9M

DOI: 10.3390/foods9091321

Abstract (EN): The volatile amines trimethylamine (TMA) and dimethylamine (DMA) could be used as important spoilage indices for seafood products, assisting in the determination of the rejection period. In the present study, a systematic analytical duality-by-design (AQbD) approach was used as a powerful strategy to optimize the most important experimental parameters of headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) conditions for the quantification of TMA and DMA inSparus aurata. This optimization enabled the selection of the best points in the method operable design region for HS-SPME extraction (30 min; 35 degrees C; NaOH 15 M and NaCl 35%,w/v) and GC-MS analysis (80 degrees C; gradient 50 degrees C/min; flow rate 1 mL/min and splitless mode). The rejection day, estimated through the TMA concentration (>12 mg/100 g, at days 9-10), was compared with sensory (quality index method: day 7-8), physical (Torrymeter: day 8-9), and microbial (day 9-10) analysis, corroborating the suitability of the proposed approach for estimating the period for which they will retain an acceptable level of eating quality from a safety and sensory perspective.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17