Methacrylated gellan gum and hyaluronic acid hydrogel blends for image-guided neurointerventions

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Vieira, S (Author) Other; Strymecka, P (Author) Other; Stanaszek, L (Author) Other; Silva Correia, J (Author) Other; Drela, K (Author) Other; Fiedorowicz, M (Author) Other; Malysz Cymborska, I (Author) Other; Rogujski, P (Author) Other; Janowski, M (Author) Other; Rui Reis (Author) Other; Lukomska, B (Author) Other; Walczak, P (Author) Other; Oliveira, JM (Author) Other

Journal

Title: Journal of Materials Chemistry B

Vol. 8

Pages: 5928-5937

ISSN: 2050-7518

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-00S-FWJ

DOI: 10.1039/d0tb00877j

Abstract (EN): Cell-based therapies deliveredviaintrathecal injection are considered as one of the most promising solutions for the treatment of amyotrophic lateral sclerosis (ALS). Herein, injectable manganese-based biocompatible hydrogel blends were developed, that can allow image-guided cell delivery. The hydrogels can also provide physical support for cells during injection, and at the intrathecal space after transplantation, while assuring cell survival. In this regard, different formulations of methacrylated gellan gum/hyaluronic acid hydrogel blends (GG-MA/HA) were considered as a vehicle for cell delivery. The hydrogels blends were supplemented with paramagnetic Mn(2+)to allow a real-time monitorization of hydrogel depositionvia T-1-weighted magnetic resonance imaging (MRI). The developed hydrogels were easily extruded and formed a stable fiber upon injection into the cerebrospinal fluid. Hydrogels prepared with a 75 : 25 GG-MA to HA ratio supplemented with MnCl(2)at 0.1 mM showed controlled hydrogel degradation, suitable permeability, and a distinct MRI signalin vitroandin vivo. Additionally, human-derived adipose stem cells encapsulated in 75 : 25 GG-MA/HA hydrogels remained viable for up to 14 days of culturein vitro. Therefore, the engineered hydrogels can be an excellent tool for injectable image-guided cell delivery approaches.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10