Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Gabriel, C (Author) Other; Liliana Grenho (Author) FMDUP; Cerqueira, F (Author) Other; Medeiros, R (Author) Other; Dias, AM (Author) Other; Ribeiro, AI (Author) Other; Proenca, MF (Author) Other; Fernandes MH (Author) FMDUP; Sousa, JC (Author) Other; Fernando Mendes Monteiro (Author) FEUP; Ferraz, MP (Author) Other

Journal

Title: Mycopathologia

Vol. 184

Pages: 775-786

ISSN: 0301-486X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00R-90J

DOI: 10.1007/s11046-019-00400-4

Abstract (EN): Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N '-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating. [GRAPHICS] .

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12