ß-casein-derived peptides, produced by bacteria, stimulate cancer cell invasion and motility

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Oliveira, MJ (Author) Other; Van Damme, J (Author) Other; Lauwaet, T (Author) Other; De Corte, V (Author) Other; De Bruyne, G (Author) Other; Verschraegen, G (Author) Other; Vaneechoutte, M (Author) Other; Goethals, M (Author) Other; Ahmadian, MR (Author) Other; Muller, O (Author) Other; Vandekerckhove, J (Author) Other; Mareel, M (Author) Other; Leroy, A (Author) Other

Journal

Title: EMBO Journal

Vol. 22

Pages: 6161-6173

ISSN: 0261-4189

Publisher: Wiley-Blackwell

Indexing

Scopus - 19 Citations

Other information

Authenticus ID: P-008-P65

DOI: 10.1093/emboj/cdg586

Abstract (EN): In colon cancer, enteric bacteria and dietary factors are major determinants of the microenvironment but their effect on cellular invasion is not known. We therefore incubated human HCT-8/E11 colon cancer cells with bacteria or bacterial conditioned medium on top of collagen type I gels. Listeria monocytogenes stimulate cellular invasion through the formation of a soluble motility-promoting factor, identified as a 13mer ß-casein-derived peptide (HKEMPFPKYP-VEP). The peptide is formed through the combined action of Mpl, a Listeria thermolysin-like metalloprotease, and a collagen-associated trypsin-like serine protease. The 13mer peptide was also formed by tumour biopsies isolated from colon cancer patients and incubated with a ß-casein source. The proinvasive 13mer peptide-signalling pathway implicates activation of Cdc42 and inactivation of RhoA, linked to each other through the serine/threonine p21-activated kinase 1. Since both changes are necessary but not sufficient, another pathway might branch upstream of Cdc42 at phosphatidylinositol 3-kinase. Delta opioid receptor (¿OR) is a candidate receptor for the 13mer peptide since naloxone, an ¿OR antagonist, blocks both ¿OR serine phosphorylation and 13mer peptide-mediated invasion.

Language: English

Type (Professor's evaluation): Scientific