Mouse HSA(+) immature cardiomyocytes persist in the adult heart and expand after ischemic injury

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Valente, M (Author) Other; Resende, TP (Author) Other; Nascimento, DS (Author) ICBAS; Burlen Defranoux, O (Author) Other; Soares da Silva, F (Author) Other; Dupont, B (Author) Other; Cumano, A (Author) Other; Perpetua Pinto do O (Author) ICBAS

Journal

Title: PLoS Biology

Vol. 17

ISSN: 1544-9173

Publisher: Public Library of Science

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00Q-SRF

DOI: 10.1371/journal.pbio.3000335

Abstract (EN): The assessment of the regenerative capacity of the heart has been compromised by the lack of surface signatures to characterize cardiomyocytes (CMs). Here, combined multiparametric surface marker analysis with single-cell transcriptional profiling and in vivo transplantation identify the main mouse fetal cardiac populations and their progenitors (PRGs). We found that CMs at different stages of differentiation coexist during development. We identified a population of immature heat stable antigen (HSA)/ cluster of differentiation 24 (CD24)(+) CMs that persists throughout life and that, unlike other CM subsets, actively proliferates up to 1 week of age and engrafts cardiac tissue upon transplantation. In the adult heart, a discrete population of HSA/CD24(+) CMs appears as mononucleated cells that increase in frequency after infarction. Our work identified cell surface signatures that allow the prospective isolation of CMs at all developmental stages and the detection of a subset of immature CMs throughout life that, although at reduced frequencies, are poised for activation in response to ischemic stimuli. This work opens new perspectives in the understanding and treatment of heart pathologies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 29