Electrochemical Characterization of Redox Probes at Gold Screen-Printed Electrodes: Efforts towards Signal Stability

• Type: Article in International Scientific Journal
• Date: 2020

Authors

José A. Ribeiro (Author) FCUP; Silva, E (Author) Other; Moreira, PS (Author) Other; Carlos M Pereira (Author) FCUP

Journal

Title: ChemistrySelect

Vol. 5

Pages: 5041-5048

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 10 Citations

Other information

Authenticus ID: P-00S-3D6

DOI: 10.1002/slct.202001411

Abstract (EN): In this work, three universally used redox probes in amperometric biosensing devices, [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-), Ru[(NH3)(6)](3+), and ferrocenedimethanol (FDM), were selected to evaluate the stability of electrochemical signals provide by the reporting systems. Studies were carried out at disposable gold screen-printed electrode (AuSPE) biosensing platforms, commonly used for screening chemical and biological relevant biomolecules. Firstly, electrochemical combined-surface plasmon resonance (eSPR) studies were performed to evaluated adsorption reversibility and/or formation of redox probe complexes at the bare gold surface when routinely used electrochemical techniques, namely cyclic voltammetry (CV) and square-wave voltammetry (SWV), are recorded. Then, the results obtained were compared with those obtained at the AuSPE under the same electrochemical conditions. Based on our findings, best experimental conditions, including the type of electrochemical technique used, are speculated for each reporting system in order to improve the analytical signal stability. Finally, a methodology based on SWV technique was applied to modified electrodes to provide a simple and easy tool to ensure diffusion controlled permeability of probes thorough the films to electrode surface.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8