Few-cycle laser for the in vitro study of cellular bioenergetics during therapeutic treatment with the anticancer drug doxorubicin in its free and liposomal nanocarrier form

• Type: Article in International Conference Proceedings Book
• Date: 2019

Authors

Ferreira, R (Author) Other; Maibohm, C (Author) Other; Silvestre, OF (Author) Other; Romero, R (Author) Other; Crespo, H (Author) FCUP; Nieder, JB (Author) Other

Conference proceedings International

Title: 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019

2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019

23 June 2019 through 27 June 2019

Indexing

Scopus - 1 Citation

Other information

Authenticus ID: P-00R-901

DOI: 10.1109/cleoe-eqec.2019.8871603

Abstract (EN): Recently we demonstrated that the endogenous NAD(P)H compounds can be used to follow the bioenergetics of cells during treatment with nanodrug delivery systems [1]. For this purpose, we exposed live cancer cells with a novel nanodrug carriers and studied the fluorescence lifetime compounds of protein-bound and unbound NAD(P)H forms, which are spectrally indistinguishable, but vary in their fluorescence lifetimes. To excite the autofluorescence of the endogenous markers we used a standard femtosecond laser source (100fs, 730nm, 80 MHz, Tsunami, Spectra Physics), which allows a two photon excitation of the NAD(P)H compounds. The fluorescence lifetime signal was detected via a time-correlated single photon counting (TCSPC) detection scheme, and the appearance of a long fluorescence lifetime component indicates the changed metabolism of the cells at longer incubation time with the therapeutic agent. © 2019 IEEE.

Language: English

Type (Professor's evaluation): Scientific