Assessment of culturable bacterial endophytic communities colonizing Canna flaccida inhabiting a wastewater treatment constructed wetland

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Coutinho Calheiros, CSC (Author) Other; Almeida Pereira, SIA (Author) Other; Brix, H (Author) Other; Santos Silva Rangel, AOSS (Author) Other; Lima Castro, PML (Author) Other

Journal

Title: Ecological Engineering

Vol. 98

Pages: 418-426

ISSN: 0925-8574

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-00M-B3T

DOI: 10.1016/j.ecoleng.2016.04.002

Abstract (EN): Microorganisms, including the bacterial populations living inside plant tissues (endophytes), and vegetation are components of constructed wetland (CW) systems playing pivotal roles in the water treatment process and in the ecosystem establishment. The present study focuses on the assessment of the culturable endophytic bacterial communities of Canna flaccida plants, the dominant plant species in a polycultured CW treating wastewater from a tourism facility. Bacterial endophytes were grouped by random amplified polymorphic DNA and identified by 16S ribosomal RNA gene sequencing. From the bacterial isolates, 103 were considered for phylogenetic analysis, falling in 25 genera within the gamma-, beta- alpha-Proteobacteria, Flavobacteria, Sphingobacteria, Actinobacteria and Bacilli classes. Forty-nine percent of the isolates belonged to the Enterobacteriaceae family, suggesting that the plants in CW systems may act as a sink of potential human pathogenic microorganisms; nevertheless their abundance on the plant tissues was reduced from the inlet (62%) to the outlet zone (38%). High diversity in terms of genera was found in C. flaccida tissues, although the number of genera common to both sampling zones was low, which suggests that the processes occurring within the CW, including the water characteristics gradient from the inlet to the outlet (e.g. total suspended solids, organics and fecal loads), had the ability to shape the diversity of the endophytic communities.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9