Hypochlorite fluorescence sensing by phenylboronic acid-alizarin adduct based carbon dots

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Simoes, EFC (Author) Other; da Silva, LP (Author) FCUP; Joaquim C G E Esteves da Silva (Author) FCUP; Leitao, JMM (Author) Other

Journal

Title: Talanta

Vol. 208

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 31 Citations

Other information

Authenticus ID: P-00R-59M

DOI: 10.1016/j.talanta.2019.120447

Abstract (EN): The selective fluorescence sensing of hypochlorite (ClO-) was achieved at pH 7.4 by a simple analytical procedure through the fluorescence quenching of autoclave synthesized carbon dots (CDs), which used as precursor an adduct formed between 3-aminophenylboronic acid (APBA) and alizarin red S (ARS). The use of this adduct allowed the preparation of CDs with a red shifted emission (560 nm) and excitation in the visible range (490 nm). Quantification of hypochlorite was achieved at physiological pH (pH 7.4) in aqueous solutions by fluorescence quenching with a linearity range of 0-200 mu M (limit of detection of 4.47 mu M, and limit of quantification of 13.41 mu M). The selectivity of hypochlorite sensing was confirmed by comparison with other potential analytes, such as glucose, fructose and hydrogen peroxide. Finally, the validity of the proposed assay was further demonstrated by performing recovery assays in different matrices. Thus, this CDs allows the fluorescent sensing of ClO- with spectral properties more suitable for in vitro/in viva applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8