Methods to Quantify DNA Transfer in Enterococcus

• Type: Chapter or Part of a Book
• Date: 2020

Authors

Novais, Carla (Author) FFUP; Freitas, AR (Author) Other; León Sampedro, R (Author) Other; Luisa Peixe (Author) FFUP; Coque, TM (Author) Other

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Scopus - 0 Citations

Other information

Authenticus ID: P-00R-51Z

DOI: 10.1007/978-1-4939-9877-7_8

Abstract (EN): DNA uptake in Enterococcus normally occurs by conjugation, a natural process that is replicated in biomedical research to assess the transferability of different mobile genetic elements and chromosomal regions as well as to study the host range of plasmids and other conjugative elements. More efficient artificial methods to transform cells with foreign DNA as chemotransformation and electroporation are widely used in molecular genetics. Here, we described conjugation protocols to quantify DNA transfer among Enterococcus and revise current perspectives and lab strains. Protocols of electrotransformation have been previously described in this series. © Springer Science+Business Media, LLC, part of Springer Nature 2020.

Language: English

Type (Professor's evaluation): Scientific