Testosterone-induced modulation of peroxisomal morphology and peroxisome-related gene expression in brown trout (Salmo trutta f. fario) primary hepatocytes

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Lopes, C (Author) Other; Malhao, F (Author) Other; Guimaraes, C (Author) Other; Pinheiro, I (Author) Other; goncalves, j (Author) ICBAS; Filipe F C Castro (Author) FCUP; rocha, e (Author) ICBAS; Madureira, TV (Author) Other

Journal

Title: Aquatic Toxicology

Vol. 193

Pages: 30-39

ISSN: 0166-445X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00N-4A2

DOI: 10.1016/j.aquatox.2017.09.026

Abstract (EN): Disruption of androgenic signaling has been linked to possible cross-modulation with other hormone-mediated pathways. Therefore, our objective was to explore effects caused by testosterone - T (1, 10 and 50 mu M) in peroxisomal signaling of brown trout hepatocytes. To study the underlying paths involved, several co-exposure conditions were tested, with flutamide - F (anti-androgen) and ICI 182,780 ICI (anti-estrogen). Molecular and morphological approaches were both evaluated. Peroxisome proliferator-activated receptor alpha (PPAR alpha), catalase and urate oxidase were the selected targets for gene expression analysis. The vitellogenin A gene was also included as a biomarker of estrogenicity. Peroxisome relative volumes were estimated by immunofluorescence, and transmission electron microscopy was used for qualitative morphological control. The single exposures of T caused a significant down-regulation of urate oxidase (10 and 50 mu M) and a general up-regulation of vitellogenin. A significant reduction of peroxisome relative volumes and smaller peroxisome profiles were observed at 50 mu M. Co-administration of T and ICI reversed the morphological modifications and vitellogenin levels. The simultaneous exposure of T and F caused a significant and concentration-dependent diminishing in vitellogenin expression. Together, the findings suggest that in the tested model, T acted via both androgen and estrogen receptors to shape the peroxisomal related targets.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10