Genome specific PPAR alpha B duplicates in salmonids and insights into estrogenic regulation in brown trout

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Madureira, TV (Author) Other; Pinheiro, I (Author) Other; Freire, RD (Author) Other; rocha, e (Author) ICBAS; Filipe F C Castro (Author) FCUP; Urbatzka, R (Author) Other

Journal

Title: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

Vol. 208

Pages: 94-101

ISSN: 0305-0491

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 10 Citations

Other information

Authenticus ID: P-00M-RHK

DOI: 10.1016/j.cbpb.2017.04.005

Abstract (EN): Peroxisome proliferator-activated receptors (PPARs) are key regulators of many processes in vertebrates, such as carbohydrate and lipid metabolism. PPAR alpha, a member of the PPAR nuclear receptor gene subfamily (NR1C1), is involved in fatty acid metabolism, namely in peroxisomal beta-oxidation. Two gene paralogues, ppar alpha A and ppar alpha B, were described in several teleost species with their origin dating back to the teleost-specific genome duplication (3R). Given the additional salmonid-specific genome duplication (4R), four genes could be theoretically anticipated for this gene subfamily. In this work, we examined the ppar alpha gene repertoire in brown trout, Salmo trutta f. fario. Data disclosed two ppar alpha-like sequences in brown trout. Phylogenetic analyses further revealed that the isolated genes are most likely genome ppar alpha B duplicates, ppar alpha Ba and ppar alpha Bb, while ppar alpha A is apparently absent in salmonids. Both genes showed a ubiquitous mRNA expression across a panel of 11 different organs. In vitro exposed primary brown trout hepatocytes strongly suggest that ppara gene paralogues are differently regulated by ethinylestradiol (EE2). ppar alpha Bb mRNA expression significantly decreased with dosage, reaching significance after exposure to 50 mu M EE2, while ppar alpha Ba mRNA increased, significant at 1 mu M EE2. The present data enhances the understanding of ppar alpha function and evolution in teleost, and reinforces the evidence of a potential crosstalk between estrogenic and ppara signaling pathways.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8