Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Lee, MC (Author) Other; Fonseca, E (Author) Other; Park, JC (Author) Other; Yoon, DS (Author) Other; Choi, H (Author) Other; Kim, M (Author) Other; Han, J (Author) Other; Cho, HS (Author) Other; Shin, KH (Author) Other; Santos, MM (Author) FCUP; Jung, JH (Author) Other; Filipe F C Castro (Author) FCUP; Lee, JS (Author) Other

Journal

Title: Environmental Science & Technology

Vol. 53

Pages: 7830-7839

ISSN: 0013-936X

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 16 Citations

Other information

Authenticus ID: P-00Q-TTD

DOI: 10.1021/acs.est.9b01359

Abstract (EN): To examine how tributyltin (TBT), a model obesogen, affects the lipid metabolism in the marine rotifer Brachionus koreanus, we carried out life-cycle studies and determined the in vitro and in silico interactions of retinoid X receptor (RXR) with TBT, the transcriptional levels of RXR and lipid metabolic genes, and the fatty acid content. The lethal concentration 10% (LC10) was determined to be 5.12 mu g/L TBT, and negative effects on ecologically relevant end points (e.g., decreased lifespan and fecundity) were detected at 5 mu g/L TBT. On the basis of these findings, subsequent experiments were conducted below 1 ug/L TBT, which did not show any negative effects on ecologically relevant end points in B. koreanus. Nile red staining analysis showed that after exposure to 1 mu g/L TBT, B. koreanus stored neutral lipids and had significantly increased transcriptional levels of RXR and lipid metabolism-related genes compared to the control. However, the content of total fatty acids did not significantly change at any exposure level. In the single fatty acids profile, a significant increase in saturated fatty acids (SFAs) 14:0 and 20:0 was observed, but the contents of omega-3 and omega-6 fatty acids were significantly decreased. Also, a transactivation assay of TBT with RXR showed that TBT is an agonist of Bk-RXR with a similar fold-induction to the positive control. Taken together, these results demonstrate that TBT-modulated RXR signaling leads to increase in transcriptional levels of lipid metabolism-related genes and the synthesis of SFAs but decreases the content of polyunsaturated fatty acids (PUFAs). Our findings support a wider taxonomic scope of lipid perturbation due to xenobiotic exposure that occurs via NRs in aquatic animals.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10