Using Cell Cultures for the Investigation of Treatments for Attention Deficit Hyperactivity Disorder: A Systematic Review

• Type: Another Publication in an International Scientific Journal
• Date: 2019

Authors

Chierrito, D (Author) Other; Villas Boas, CB (Author) Other; Tonin, FS (Author) Other; Fernandez Llimos, F (Author) Other; Sanches, ACC (Author) Other; de Mello, JCP (Author) Other

Journal

Title: Current Neuropharmacology

Vol. 17

Pages: 916-925

ISSN: 1570-159X

Publisher: Bentham Science

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-00R-3Q9

DOI: 10.2174/1570159x17666190409143155

Abstract (EN): Background: Advances in basic and molecular biology have promoted the use of cell cultures in a wide range of areas, including the evaluation of drug efficacy, safety and toxicity. Objective This article aims to provide a general overview of the methodological parameters of cell cultures used to investigate therapeutic options for Attention Deficit Hyperactivity Disorder. Method: A systematic search was performed in the electronic databases PubMed, Scopus, and DOAJ. In vitro experimental studies using cell cultures were included. Results: A total of 328 studies were initially identified, with 16 included for qualitative synthesis. Seven studies used neuronal cells (SH-SY5Y neuroblastoma and PC12 cell line) and nine used non-neuronal cells. All the studies described the culture conditions, but most studies were inconsistent with regard to reporting results and raw data. Only one-third of the studies performed cell viability assays, while a further 30% conducted gene expression analysis. Other additional tests included electrophysiological evaluation and transporter activity. More than 50% of the studies evaluated the effects of drugs such as methylphenidate and atomoxetine, while plant extracts were assessed in four studies and polyunsaturated fatty acids in one. Conclusion: We suggested a flowchart to guide the planning and execution of studies, and a checklist to be completed by authors to allow the standardized reporting of results. This may guide the elaboration of laboratory protocols and further in vitro studies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10