Vitek (R) MS v3.0 System in the Identification of Filamentous Fungi

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Pinheiro, D (Author) Other; Monteiro, C (Author) Other; Faria, MA (Author) Other; Eugenia Pinto (Author) FFUP

Journal

Title: Mycopathologia

Vol. 184

Pages: 645-651

ISSN: 0301-486X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-00R-204

DOI: 10.1007/s11046-019-00377-0

Abstract (EN): Infections caused by filamentous fungi are rising in incidence and became a serious health concern. Their rapid and reliable identification in the clinical laboratory is essential for an early and accurate diagnosis to guide timely therapy. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a rapid and reliable method for identification of bacteria and yeasts isolated from clinical samples. However, it has less used for molds identification. The aim of this study was to evaluate Vitek (R) MS (a MALDI-TOF MS system) ability to identify molds and differentiate species within a complex. A collection of 90 filamentous fungi, 70 clinical and 20 environmental isolates, was studied by morphological and molecular methods and by Vitek MS. Seventy-four isolates (82.2%) were identified using Vitek (R) MS v3.0 at Genus/Complex/Species group level; within these, 47/74 (63.5%) were correctly identified at species level and only one was misidentified. In contrast, 16/90 isolates (17.8%) were not identified, of which 13 were not present in the database. Results here expressed favor Vitek MS v3.0 as a very useful system for identification of most common clinical isolates of filamentous fungi. Accordingly, it may be an important tool for clinical microbiology laboratories in their task to answer to clinicians, adequately and rapidly, helping in proper patient's management.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7