Cow's milk allergens: Screening gene markers for the detection of milk ingredients in complex meat products

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Villa, C (Author) Other; Joana Costa (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP; Mafra, I (Author) Other

Journal

Title: Food Control

Vol. 108

ISSN: 0956-7135

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-00Q-ZKB

DOI: 10.1016/j.foodcont.2019.106823

Abstract (EN): Cow's milk proteins are food allergens present in uncounted foodstuffs. By exploiting and comparing different regions of bovine genome, this work intended to develop a real-time PCR method to detect/quantify traces of milk in complex foods. Two mitochondrial (cytb and 12S rRNA) and two nuclear (beta-lactoglobulin and beta-casein) genes showed the most promising results by qualitative PCR. However, real-time PCR assays targeting nuclear genes revealed cross-reactivity with some meat species, while the 12S rRNA assay was the most specific (only minor reactivity with two species above 36 cycles of amplification), providing a linear dynamic range of 10-0.05% of cow's milk protein concentrate (MPC) in raw meat and cooked ham, with acceptable performance parameters. The results highlight the potentiality of the assay for the development of a novel method to quantify milk ingredients in meat products.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8