Enantioseparation, recognition mechanisms and binding of xanthones on human serum albumin by liquid chromatography

• Type: Article in International Scientific Journal
• Date: 2019

Authors

do Carmo, JP (Author) Other; Phyo, YZ (Author) Other; Palmeira, A (Author) FFUP; M E Tiritan (Author) FFUP; Carlos Afonso (Author) FFUP; Kijjoa, A (Author) Other; Pinto, MMM (Author) Other; Carla Fernandes (Author) FFUP

Journal

Title: Bioanalysis

Vol. 11

Pages: 1255-1274

ISSN: 1757-6180

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-00R-1DC

DOI: 10.4155/bio-2019-0074

Abstract (EN): Aim: To develop a method for enantioseparation of several chiral derivatives of xanthones (CDXs) by LC using a human serum albumin-chiral stationary phase (HSA-CSP) and screening CDX-HSA affinity. Additionally, recognition mechanisms were investigated. Materials & methods: The influence of organic modifier, buffer type, pH and ionic strength of mobile phase, and temperature were explored. The affinity was determined by measuring the retention times and further calculation of bound percentage. Chiral recognition mechanisms were investigated by docking. Results: Enantioselectivity and resolution values ranged from 1.40 to 9.16 and 1.51 to 4.97. Bound percentages ranged from 79.02 to 99.99%. Conclusion: LC systematic study and binding affinity of CDXs on HSA-CSP are presented here for the first time, expanding the applications of HSA-CSP for this class of compounds.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20