Diagnostic value of CD103 expression in bronchoalveolar lymphocytes in sarcoidosis

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Mota, PC (Author) Other; Morais, A (Author) Other; Palmares, C (Author) Other; Beltrao, M (Author) Other; Melo, N (Author) Other; Santos AC (Author) FMUP; Delgado, L (Author) FMUP

Journal

Title: Respiratory Medicine

Vol. 106

Pages: 1014-1020

ISSN: 0954-6111

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-002-8NV

DOI: 10.1016/j.rmed.2012.03.020

Abstract (EN): Background: Pulmonary sarcoidosis is frequently characterized by a CD4(+)/CD8(+) ratio >= 3.5 in bronchoalveolar lavage fluid (BALF), although up to 40% of the cases present a normal or even decreased ratio, pointing out its variability and limitation as a diagnostic marker for sarcoidosis. Lung lymphocytes within the bronchial epithelium, the alveolar walls, and BALF express the integrin CD103. Our aim was to compare the expression of CD103 in BALE T-lymphocytes between sarcoidosis and other interstitial lung diseases (ILD) and to evaluate its relevance as a BALF diagnostic marker for sarcoidosis. Methods: A total of 86 patients with ILD (mean age +/- standard deviation, 42.6 +/- 16.6 years; 60.5% female), who underwent BALE as part of their initial diagnostic work-up, were enrolled into 2 groups: sarcoidosis (n = 41) and other ILD (n = 45). Area under the receiver operating characteristic (ROC) curve (AUC) was used to describe the performance of CD103 for sarcoidosis diagnosis. Results: Sarcoidosis patients presented a significantly reduced CD103 expression in BALE T-lymphocytes, more pronounced in the CD4(+) subset. The BALF CD103(+)CD4(+)/CD4(+) ratio for a cutoff point of 0.45 was associated with a better diagnostic performance for sarcoidosis (AUC: 0.86 [95% confidence interval (95% Cl): 0.78-0.94]; sensitivity: 81%; specificity: 78%), even for those with a CD4(+)/CD8(+) ratio <3.5 (AUC: 0.79 [95% Cl: 0.64-0.93]; sensitivity: 75%; specificity: 78%). Conclusions: Assessment of CD103 expression in BALECD4+ T-lymphocytes may be a reliable tool for sarcoidosis diagnosis, independently of CD4(+)/CD8(+) ratio, pointing out the relevance of evaluating the CD103(+)CD4(+)/CD4(+) ratio in the ILD diagnostic work-up.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7