Vitamin A Enhances Macrophages Activity Against B16-F10 Malignant Melanocytes: A New Player for Cancer Immunotherapy?

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Oliveira, S (Author) Other; Costa, J (Author) Other; Faria, I (Author) Other; Susana Guerreiro (Author) FCNAUP; Fernandes, R (Author) Other

Journal

Title: Medicina-Lithuania

Vol. 55

ISSN: 1010-660X

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences

Other information

Authenticus ID: P-00R-35C

DOI: 10.3390/medicina55090604

Abstract (EN): Background and objectives: The incidence of cutaneous melanoma has been increasing. Melanoma is an aggressive form of skin cancer irresponsive to radiation and chemotherapy, rendering this cancer a disease with poor prognosis: In order to surpass some of the limitations addressed to melanoma treatment, alternatives like vitamins have been investigated. In the present study, we address this relationship and investigate the possible role of vitamin A. Materials and Methods: We perform a co-culture assay using a macrophage cell model and RAW 264.7 from mouse, and also a murine melanoma cell line B16-F10. Macrophages were stimulated with both Escherichia coli lipopolysaccharides (LPS) as control, and also with LPS plus vitamin A. Results: Using B16-F10 and RAW 264.7 cell lines, we were able to demonstrate that low concentrations of vitamin A increase cytotoxic activity of macrophages, whereas higher concentrations have the opposite effect. Conclusion: These findings can constitute a new point of view related to immunostimulation by nutrients, which may be considered one major preventive strategy by enhancing the natural defense system of the body.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9