Enzymatic Reactions in a Lab-on-Valve System: Cholesterol Evaluations

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Barbosa, JS (Author) Other; Passos, MLC (Author) Other; Korn, MDA (Author) Other; Lucia L M F S Saraiva (Author) FFUP

Journal

Title: Molecules

Vol. 24

Final page: 2890

ISSN: 1420-3049

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00Q-XWD

DOI: 10.3390/molecules24162890

Abstract (EN): The micro sequential injection analysis / lab-on-valve (mu SIA-LOV) system is a miniaturized SIA system resulting from the implementation of a lab-on-valve (LOV) atop of the selection valve. It integrates the detection cell and the sample processing channels into the same device, promoting the reduction of reagent consumption and waste generation, the improvement of the versatility, and the reduction of the time of analysis. All of these characteristics are really relevant to the implementation of enzymatic reactions. Additionally, the evaluation of cholesterol in serum samples is widely relevant in clinical diagnosis, since higher values of cholesterol in human blood are actually an important risk factor for cardiovascular problems. An automatic methodology was developed based on the mu SIA-LOV system in order to evaluate its advantages in the implementation of enzymatic reactions performed by cholesterol esterase, cholesterol oxidase and peroxidase. Considering these reactions, the developed methodology was also used for the evaluation of cholesterol in human serum samples, showing reliable and accurate results. The developed methodology presented detection and quantification limits of 1.36 and 4.53 mg dL(-1) and a linear range up to 40 mg dL(-1). This work confirmed that this mu SIA-LOV system is a simple, rapid, versatile, and robust analytical tool for the automatic implementation of enzymatic reactions performed by cholesterol esterase, cholesterol oxidase, and peroxidase. It is also a useful alternative methodology for the routine determinations of cholesterol in real samples, even when compared with other automatic methodologies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12