Online HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from Moringa oleifera leaves

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Braham, F (Author) Other; Carvalho, DO (Author) FCUP; Marisa M R Almeida (Author) FCUP; Zaidi, F (Author) Other; Magalhaes, JMCS (Author) Other; Luis F Guido (Author) FCUP; Goncalves, MP (Author) Other

Journal

Title: South African Journal of Botany

Vol. 129

Pages: 146-154

ISSN: 0254-6299

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-00Q-KDM

DOI: 10.1016/j.sajb.2019.04.001

Abstract (EN): In this work the phenolic profile of Moringa oleifera leaves from trees located in Oued Souf and Tamanrasset, desert region south Algeria, was characterized by a real-time HPLC coupled to the DPPH assay, together with high resolution mass spectrometry (HPLC-DAD-ESI-MSn) studies. The contribution of single component to the overall radical scavenging capacity was evaluated for water, hydroalcoholic and hydroacetonic extracts of M. oleifera leaves. Hydroacetonic extracts (70% acetone) exhibit the highest amount of phenolic compounds (57 +/- 3 mg GAE/g), as well as the highest flavonoid content (9.12 +/- 0.01 mg QE/g). Twelve compounds, mainly phenolics, were identified by high-resolution orbitrapmass spectrometry. Among these, chlorogenic acids (3-caffeoylquinic acid and 4-caffeoylquinic acid) and quercetin glycosides (quercetin-3-O-glucoside, quercetin-3-O-galactoside and quercetin 3-O-(6 ''-malonyl) glucoside) are the most potent quenchers of DPPH radical both for hydroethanolic and hydroacetonic extracts. Aqueous extracts exhibit a quite different behaviour, with citric acid and quinic acid, arising from the oxidation of the chlorogenic acids, acting as the unique antioxidants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9