Virulence, attachment and invasion of Caco-2 cells by multidrug-resistant bacteria isolated from wild animals

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Carla Dias (Author) Other; Marta Ribeiro (Author) Other; Ana Correia Branco (Author) Other; Raul Domínguez-Perles (Author) Other; Fátima Martel (Author) Other; Maria José Saavedra (Author) Other; Manuel Simões (Author) FEUP

Journal

Title: Microbial Pathogenesis

Vol. 128

Pages: 230-235

ISSN: 0882-4010

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 7 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00Q-AKS

DOI: 10.1016/j.micpath.2019.01.011

Abstract (EN): Wild animals may be considered important reservoirs for bacterial pathogens and, consequently, possible sources of infection for humans In this study, selected multidrug-resistant bacteria (Acinetobacter spp., Aeromonas salmonicida, Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from wild animals were characterized on their ability to attach and invade/internalize human colonic carcinoma (Caco-2) cells. In addition, the viability of these bacteria to survive under simulated human gastrointestinal tract conditions as well as the production of virulence factors (homoserine lactones signal molecules, gelatinases, proteases, siderophores and biofilm formation) were studied. The results suggests that all the bacteria presented the capacity to attach and internalize into Caco-2 cells. A. salmonicida and P. fluorescens exhibited the highest ability to internalize. These bacteria were also found to be the highest proteases producers. A. salmonicida and K. pneumoniae survived under simulated human gastrointestinal conditions. These were the bacteria with the highest capacity to produce biofilms. K. pneumoniae was the only bacterium producing siderophores. Taken together, the present results reinforce the need for the "One Health" initiative, underscoring the environment and the animals as important reservoirs of infectious determinants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6