Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Alex Fiori Silva (Author) Other; Adriele Rodrigues dos Santos (Author) Other; Daliah Alves Coelho Trevisan (Author) Other; Edineia Bonin (Author) Other; Camila Fabiano Freitas (Author) Other; Andreia Farias Pereira Batista (Author) Other; Noboru Hioka (Author) Other; Manuel Simões (Author) FEUP; Jane Martha Graton Mikcha (Author) Other

Journal

Title: Photochemistry and Photobiology

Vol. 95 No. 5

Pages: 1230-1238

ISSN: 0031-8655

Publisher: Wiley-Blackwell

Indexing

ISI Web of Science

Scopus

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

DOI: 10.1111/php.13104

Abstract (EN): This study evaluated the rose bengal- and erythrosine-mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free-living or 2-day-old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L-1 and 75 mu mol L-1 completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L-1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 mu mol L-1 of erythrosine or 250 mu mol L-1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9