Disposable electrochemical detection of breast cancer tumour marker CA 15-3 using poly(Toluidine Blue) as imprinted polymer receptor

• Type: Article in International Scientific Journal
• Date: 2018

Authors

José A. Ribeiro (Author) FCUP; Carlos M Pereira (Author) FCUP; Silva, F (Author) FCUP; Sales, MGF (Author) Other

Journal

Title: Biosensors and Bioelectronics

Vol. 109

Pages: 246-254

ISSN: 0956-5663

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 90 Citations

Scopus - 100 Citations

Other information

Authenticus ID: P-00N-S0F

DOI: 10.1016/j.bios.2018.03.011

Abstract (EN): In this work, electrically-conducting poly(Toludine Blue) was employed for the first time as synthetic receptor film, prepared by Molecular Imprinting strategies and using electrochemical methods, for the specific screening of breast cancer biomarker Carbohydrate Antigen 15.3 (CA 15-3). The protein imprinted poly(Toluidine Blue) film was grown in a pre-formed Toluidine Blue (TB) tailed SAM at the AuSPE surface, which greatly enhanced the stability against degradation of the Molecular Imprinted Polymer (MIP) film at the electrode surface. The MIP receptor film recognition ability towards the protein was investigated by fitting data to Freundlich isotherm. The binding affinity (K-p) obtained for the MIP system was significantly higher (similar to 12-fold) to that obtained for the NIP system, demonstrating the success of the approach in creating imprinted materials that specifically respond to CA 15-3 protein. The incubation of the MIP modified electrode with increasing concentration of protein (from 0.10 U mL(-1) to 1000 U mL(-1)) resulted in a decrease of the ferro/ferricyanide redox current. The device displayed linear response from 0.10 U mL(-1) to 100 U mL(-1) and LODs below 0.10 U mL(-1) were obtained from calibration curves built in neutral buffer and diluted artificial serum, using DPV technique, enabling the detection of the protein biomarker at clinically relevant levels. The developed MIP biosensor was applied to the determination of CA 15-3 in spiked serum samples with satisfactory results. The developed device provides a new strategy for sensitive, rapid, simple and cost-effective screening of CA 15-3 biomarker. Importantly, the overall approach seems suitable for point-of-care (PoC) use in clinical context.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9