GST transcriptional changes induced by a toxic Microcystis aeruginosa strain in two bivalve species during exposure and recovery phases

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Antas, P (Author) Other; Carneiro, M (Author) Other; Reis, B (Author) Other; Castelo Branco, R (Author) Other; Azevedo, J (Author) Other; Urbatzka, R (Author) Other; Alexandre Campos (Author) Other; Vitor Vasconcelos (Author) FCUP; Martins, JC (Author) Other

Journal

Title: Ecotoxicology

Vol. 27

Pages: 1272-1280

ISSN: 0963-9292

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00P-P2W

DOI: 10.1007/s10646-018-1980-y

Abstract (EN): Previous studies have demonstrated the modulation of glutathione transferases (GSTs) induced by microcystin (MC) alone or in combination with other cyanobacterial secondary metabolites in bivalves. However, interspecies information about which and how GST isoforms are affected by these secondary metabolites is still scarce, especially considering the dynamic process involving their uptake and elimination routes. In this context, the role of GSTs gene expression changes in response to a toxic Microcystis aeruginosa extract were examined for Mytilus galloprovincialis and Ruditapes philippinarum during exposure and recovery phases. The expression levels of sigma 1, sigma 2, pi and mu-class GST genes were analyzed in the hepatopancreas of both bivalve species during cyanobacteria extract exposure (24h) and post-exposure (24 and 72h). Only a significant induction of sigma 1-class GST expression was observed for R. philippinarum upon 24-hour exposure of both bivalve species to Microcystis extract. During the recovery phase, GST transcriptional changes for M. galloprovincialis were characterized by an early induction (24h) of sigma 1 and sigma 2 transcripts. On the other hand, GST transcriptional changes for R. philippinarum during post-exposure phase were characterized by an early induction (24h) of sigma 1 and mu transcripts and a later induction (72h) of the four analyzed GST transcripts. Such differences reflect variable GST response mechanisms to cope with MC-producing cyanobacterial blooms exposure between these two bivalve species, revealing a higher sensitivity of R. philippinarum to Microcystis-induced stress than M. galloprovincialis. The results also suggest a much higher level of activity of the GST detoxification system during the recovery phase compared to the period of the stress exposure for both bivalve species.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9