Neofiscalin A and fiscalin C are potential novel indole alkaloid alternatives for the treatment of multidrug-resistant Gram-positive bacterial infections

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Bessa, LJ (Author) Other; Buttachon, S (Author) Other; Dethoup, T (Author) Other; Martins, R (Author) Other; Vitor Vasconcelos (Author) FCUP; Kijjoa, A (Author) Other; da Costa, PM (Author) Other

Journal

Title: FEMS Microbiology Letters

Vol. 363

ISSN: 0378-1097

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 27 Citations

Scopus - 27 Citations

Other information

Authenticus ID: P-00K-QM0

DOI: 10.1093/femsle/fnw150

Abstract (EN): Ten indole alkaloids were obtained from the marine sponge-associated fungus Neosartorya siamensis KUFA 0017. We studied the antimicrobial properties of these and of three other compounds previously isolated from the soil fungus N. siamensis KUFC 6349. Only neofiscalin A showed antimicrobial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE); with a minimum inhibitory concentration (MIC) of 8 mu g mL(-1) against both strains. Another compound, fiscalin C, presented synergistic activity against MRSA when combined with oxacillin, although alone showed no antibacterial effect. Moreover, neofiscalin A, when present at sub-MICs, hampered the ability of both MRSA and VRE strains to form a biofilm. Additionally, the biofilm inhibitory concentration values of neofiscalin A against the MRSA and VRE isolates were 96 and 80 mu g mL(-1), respectively. At a concentration of 200 mu g mL(-1), neofiscalin A was able to reduce the metabolic activity of the biofilms by similar to 50%. One important fact is that our results also showed that neofiscalin A had no cytotoxicity against a human brain capillary endothelial cell line.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5