Cardiolipin and phosphatidylethanolamine role in dibucaine interaction with the mitochondrial membrane

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Lopes, SC (Author) Other; Ivanova, G (Author) Other; de Castro, B (Author) FCUP; Paula Gameiro (Author) FCUP

Journal

Title: Biochimica et Biophysica Acta: Biomembranes

Vol. 1861

Pages: 1152-1161

ISSN: 0005-2736

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00Q-F5S

DOI: 10.1016/j.bbamem.2019.02.011

Abstract (EN): Mitochondrial membranes are pointed out as the site of cardiotoxic action of local anaesthetics. Its three main phospholipids components are phosphatidylcholine, phosphatidylethanolamine and cardiolipin. Cardiolipins, in eukaryotes, are only found in mitochondria and are essential for the maintenance of its integrity and dynamics. Fluorescence and nuclear magnetic resonance spectroscopy were used to study the interactions of a local anaesthetics, Dibucaine (DBC), with different mitochondrial membrane models constituted by combinations of its three main lipid components in which cardiolipin was a natural extract (CLmix). Both CLmix presence/absence and its percentage in the model membranes were evaluated. Fluorescence spectroscopy showed that DBC lowered the transition temperature of all membrane models understudy. DBC partition showed to be dependent of CLmix presence and phosphatidylethanolamine:CL ratio. Furthermore, the maximum emission wavelength (lambda(max)) exhibited a notorious decreased with increasing phospholipid to DBC ratio, in all the membrane models containing CLmix. Nevertheless, it remained approximately the same in the membrane without CLmix. This indicates a differential membrane localization of the anaesthetics, dependent on the membrane models used. NMR results showed that DBC interaction and location in the membrane models is mainly influenced by CLmix presence, and DBC can significant alter lipid systems properties e.g. percentage and type of lipid phase present. Taken all together it was shown that DBC interaction and location are largely dependent on the membrane model system. Furthermore, DBC is able to produce significant changes in the lipidic systems which might help to explain its high toxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10