Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Bessa, LJ (Author) Other; Eaton, P (Author) Other; Dematei, A (Author) Other; Placido, A (Author) Other; Nuno Vale (Author) ICBAS; Paula Gomes (Author) FCUP; Delerue Matos, C (Author) Other; Leite, JRSA (Author) Other; Paula Gameiro (Author) FCUP

Journal

Title: Future Microbiology

Vol. 13

Pages: 151-163

ISSN: 1746-0913

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 45 Citations

Scopus - 49 Citations

Other information

Authenticus ID: P-00N-REB

DOI: 10.2217/fmb-2017-0175

Abstract (EN): To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8x the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa. [GRAPHICS] .

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13