A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki ( Brush- Foot Trapdoor)

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Undheim, EAB (Author) Other; Sunagar, K (Author) Other; Herzig, V (Author) Other; Kely, L (Author) Other; Low, DHW (Author) Other; Jackson, TNW (Author) Other; Jones, A (Author) Other; Kurniawan, N (Author) Other; King, GF (Author) Other; Ali, SA (Author) Other; Agostinho Antunes (Author) FCUP; Ruder, T (Author) Other; Fry, BG (Author) Other

Journal

Title: Toxins

Vol. 5

Pages: 2488-2503

ISSN: 2072-6651

Publisher: MDPI

Indexing

ISI Web of Knowledge - 55 Citations

Scopus - 57 Citations

Other information

Authenticus ID: P-008-K4C

DOI: 10.3390/toxins5122488

Abstract (EN): Although known for their potent venom and ability to prey upon both invertebrate and vertebrate species, the Barychelidae spider family has been entirely neglected by toxinologists. In striking contrast, the sister family Theraphosidae (commonly known as tarantulas), which last shared a most recent common ancestor with Barychelidae over 200 million years ago, has received much attention, accounting for 25% of all the described spider toxins while representing only 2% of all spider species. In this study, we evaluated for the first time the venom arsenal of a barychelid spider, Trittame loki, using transcriptomic, proteomic, and bioinformatic methods. The venom was revealed to be dominated by extremely diverse inhibitor cystine knot (ICK)/knottin peptides, accounting for 42 of the 46 full-length toxin precursors recovered in the transcriptomic sequencing. In addition to documenting differential rates of evolution adopted by different ICK/knottin toxin lineages, we discovered homologues with completely novel cysteine skeletal architecture. Moreover, acetylcholinesterase and neprilysin were revealed for the first time as part of the spider-venom arsenal and CAP (CRiSP/Allergen/PR-1) were identified for the first time in mygalomorph spider venoms. These results not only highlight the extent of venom diversification in this neglected ancient spider lineage, but also reinforce the idea that unique venomous lineages are rich pools of novel biomolecules that may have significant applied uses as therapeutics and/or insecticides.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16