Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Wegelius, A (Author) Other; Khanna, N (Author) Other; Esmieu, C (Author) Other; Barone, GD (Author) Other; Pinto, F (Author) Other; Paula Tamagnini (Author) FCUP; Berggren, G (Author) Other; Lindblad, P (Author) Other

Journal

Title: Energy and Environmental Sciences

Vol. 11

Pages: 3163-3167

ISSN: 1754-5692

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 35 Citations

Other information

Authenticus ID: P-00P-WR5

DOI: 10.1039/c8ee01975d

Abstract (EN): [FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H-2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5