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Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers

Title
Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers
Type
Article in International Scientific Journal
Year
2016
Authors
Grajeda Iglesias, C
(Author)
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Figueroa Espinoza, MC
(Author)
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Barouh, N
(Author)
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Barea, B
(Author)
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Fernandes, A
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Victor de Freitas
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Salas, E
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Journal
Vol. 79
Pages: 1709-1718
ISSN: 0163-3864
Other information
Authenticus ID: P-00K-P21
Abstract (EN): The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C-18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid base equilibrium (pK(a)' 2.92), the acidity (pK(a) 3.70), and the hydration constants (pK(h) 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 10
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