Electrochemical sensing of the thyroid hormone thyronamine (T(0)AM) via molecular imprinted polymers (MIPs)

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Pacheco, JG (Author) Other; Rebelo, P (Author) Other; Fernando Cagide (Author) FCUP; Goncalves, LM (Author) Other; Fernanda Borges (Author) FCUP; Rodrigues, JA (Author) FCUP; Delerue Matos, C (Author) Other

Journal

Title: Talanta

Vol. 194

Pages: 689-696

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 30 Citations

Other information

Authenticus ID: P-00P-VDG

DOI: 10.1016/j.talanta.2018.10.090

Abstract (EN): Recent studies have shown that besides the well-known T-3 (triiodothyronine) and T-4 (thyroxine) there might be other important thyroid hormones, in particular T(0)AM (thyronamine) and T(1)AM (3-iodothyronamine). The absence of a large number of studies showing their precise importance might be explained by the limited number of analytical methodologies available. This work aims to show an electroanalytical alternative making use of electropolymerized molecularly imprinted polymer (MIPs). The MIPs' polymerization is performed on the surface of screen-printed carbon electrodes (SPCEs), using 4-aminobenzoic acid (4-ABA) as the building and functional monomer and the analyte T(0)AM as the template. The step-by-step construction of the SPCE-MIP sensor was studied by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS). After optimization, by means of square-wave voltammetry, the SPCE-MIP showed suitable selectivity (in comparison with other thyroid hormones and catechol amines), repeatability (intra-day of 3.9%), a linear range up to 10 mu mol L-1 (0.23 x 10(3) mu g dL(-1)) with an r(2) of 0.998 and a limit of detection (LOD) and quantification (LOQ) of 0.081 and 0.27 mu mol L-1 (L9 and 6.2 mu g dL(-1)), respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8