HEAT-SHOCK EFFECT ON GLUTATHIONE AND SUPEROXIDE-DISMUTASE IN TETRAHYMENA-PYRIFORMIS

• Type: Article in International Scientific Journal
• Date: 1992

Authors

MORADAS, FP (Author) Other; Vitor Costa (Author) ICBAS; GUERREIRO, P (Author) Other; RODRIGUES, PC (Author) Other

Journal

Title: Cell Biology International Reports

Vol. 16

Pages: 19-26

ISSN: 0309-1651

Publisher: Academic Press

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-001-PZR

Abstract (EN): Intracellular levels of total glutathione and cytosolic superoxide dismutase activity were assayed in cells from Tetrahymena pyriformis either exposed to sub-lethal (34°C) or to lethal heat shock (39°C). The results showed that glutathione levels decrease to 60% of normal values after a sub-lethal heat shock for 1 hour. This change is part of the heat shock response, since the effect is reversed as soon as cells are brought to their normal growing temperature. Using actinomycin D, which blocks the synthesis of high molecular weight hsp (Galego and Rodrigues-Pousada, 1985), prior to thermal stress, the fall in total glutathione is not observed, suggesting a partial correlation with the synthesis of these stress proteins. Using cells pre-exposed to a sub-lethal heat shock, a subsequent short severe heat shock does not lead to a significant decrease of the glutathione content. Superoxide dismutase (SOD) activity is not significantly induced after either a short period at 34°C or a prolonged treatment at the same temperature. © 1992 Academic Press Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8