River water analysis using a multiparametric approach: Portuguese river as a case study

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Barbosa Vasconcelos, A (Author) Other; Mendes, A (Author) Other; Martins, F (Author) Other; Lopes, E (Author) Other; Machado, A (Author) Other; Bordalo, AA (Author) Other; Vaz Pires, P. (Author) ICBAS; Vieira, MN (Author) FCUP; da Costa, PM (Author) Other; Bessa, LJ (Author) Other

Journal

Title: Journal of Water and Health

Vol. 16

Pages: 991-1006

ISSN: 1477-8920

Publisher: IWA PUBLISHING

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00Q-0FQ

DOI: 10.2166/wh.2018.047

Abstract (EN): The Ave River in northern Portugal has a history of riverbanks and water quality degradation. The river water quality was assessed by physicochemical, biological (macroinvertebrates) and microbiological (Enterococcus spp. and Escherichia coli) parameters in six locations (A-F, point A being the nearest to the source) throughout its course during a year. Epilithic biofilms were studied through polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). Antimicrobial susceptibility testing helped with selecting isolates (n = 149 E. coli and n = 86 enterococci) for further genetic characterization. Pursuant to physicochemical and macroinvertebrates-based parameters, the river water was of reasonable quality according to European legislation (Directive 2000/60/EC). However, the microbiological analysis showed increased fecal contamination downstream from point C. At point D, four carbapenem-resistant E. coli isolates were recovered. Paradoxically, point D was classified as a point of 'Good Water Quality' according to macroinvertebrates results. Point F presented the highest contamination level and incidence of multidrug-resistant (MDR) isolates in the water column (13 MDR enterococci out of 39 and 33 MDR E. coli out of 97). Epilithic biofilms showed higher diversity in pristine points (A and B). Thus, biological and microbiological parameters used to assess the water quality led to divergent results; an outcome that reinforces the need for a holistic evaluation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16