Morphological and biochemical responses of maize calli to UV-C rays: Interaction of UV-C radiation with ion content and peroxidase and alpha-amylase activity

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Brito, G (Author) Other; Lopes, VC (Author) Other; Caldeira, GN (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

Vol. 61

Pages: 37-44

ISSN: 0031-9457

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-001-CV3

Abstract (EN): Maize (Zea mays L. cv. Colt) calli were induced and grown on modified MS medium. Calli were irradiated during seven days with UV-C light (3.170 mWcm(-2)) for 15 min. Most irradiated calli became dark, with burnt necrotic areas, but from those regions in which growth was maintained, a white friable tissue developed. Some weeks after UV-irradiation, regeneration of healthy white roots from the new calli was observed. UV-C irradiation decreased by 50% callus fresh weight after 15 days. Irradiated calli showed a decrease in K+ and Ca2+ and L-proline content, whereas soluble protein levels increased. In irradiated cells, peroxidase activity decreased but a-amylase activity was stimulated.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8