Interferon-gamma-dependent protection against Neospora caninum infection conferred by mucosal immunization in IL-12/IL-23 p40-deficient mice

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Ferreirinha, P (Author) Other; Frois Martins, R (Author) Other; Teixeira, L (Author) ICBAS; Rocha, A (Author) Other; Manuel Vilanova (Author) ICBAS; Alexandra Correia (Author) ICBAS

Journal

Title: Vaccine

Vol. 36

Pages: 4890-4896

ISSN: 0264-410X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00P-GJX

DOI: 10.1016/j.vaccine.2018.06.060

Abstract (EN): We have recently demonstrated the effectiveness of an intranasal immunization approach against Neospora caninum infection in immunosufficient mice. Generated evidence indicated that antibodies could be mediating the observed protection. We similarly immunized IL-12/IL-23 p40 chain-deficient (Il12b(-/-)) mice, which have impaired cellular immunity, to further explore the host protective mechanism conferred by the used immunization strategy. The immunized mice presented lower parasitic burdens after intraperitoneal infection with N. caninum and also had elevated levels of parasite-specific antibodies. However, passive immunization with antibodies purified from immunized donors conferred only limited protection to infected Il12b(-/-) recipients. Despite their intrinsic IL-12 deficiency, the immunized Il12b(-/-) mice mounted a parasite-specific immune response that was mediated by interferon-gamma (IFN-gamma). Neutralization of IFN-gamma in the immunized mice abrogated the observed protective effect of the immunization. These results show altogether that the used immunization strategy overcome the cellular immunity defect of Il12b(-/- )mice and conferred protection from N. caninum infection. The observed protective effect was predominantly mediated by IFN-gamma and to a lesser extent but non-negligibly by IgG antibodies. These results also highlight that in a host with compromised cellular immunity, the immune response against intracellular pathogens could be markedly boosted by immunization.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7