The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study

• Type: Article in International Scientific Journal
• Date: 2015-01-01

Authors

Lopes, MA (Author) FEUP; J. Torres (Author) Other; Santos, JD (Author) FEUP; Gutierres, M (Author) Other; Cabral, AT (Author) Other; Fernandes MH (Author) Other; Monteiro, E (Author) Other; van Eck, CF (Author) Other; Lopes A (Author) Other

Journal

Title: BioMed Research International

Vol. 2015 No. ID 531789

Pages: 1-10

ISSN: 2314-6133

Publisher: Hindawi

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Pubmed / Medline

Other information

Authenticus ID: P-00A-5X7

DOI: 10.1155/2015/240698

Abstract (EN): Background. This work compares the osteoblastic behaviour of a bone marrow (BM) aspirate and a prepared BM concentrate of nucleated cells associated with a glass reinforced hydroxyapatite composite (GRHC) in a microporous pellet formulation. Methods. BM aspirate (30 mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3 mL) from the BM aspirates. The BM aspirates (53% cell viability; 2.7x10(6) nucleated cell/mL) and the BM concentrates (76% cell viability; 2x10(7) nucleated cell/mL) were cultured over glass reinforced hydroxyapatite pellets, at the same volume/mass ratio, for 30 days. Cultures performed in standard tissue culture plates were used as control. Results. The colonized BM concentrate/material constructs exhibited a representative osteoblastic proliferation/differentiation pathway, evidenced by a high alkaline phosphatase (ALP) activity, expression of collagen type 1, ALP, BMP-2, M-CSF, RANKL, and OPG, and formation of a calcium phosphate mineralized matrix. A clear improved behaviour was noticed compared to the BM aspirate/material constructs. Conclusions. The results suggest the benefit of using an autologous BM concentrate/material construct in the clinical setting, in bone regeneration applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10