Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

• Type: Chapter or Part of a Book
• Date: 2017

Authors

Sílvia J. Fontenete (Author) Other; Mariana Leite (Author) Other; Céu Figueiredo (Author) Other; Paul Cos (Author) Other; Nuno Filipe Azevedo (Author) FEUP; Rita Santos (Author) FEUP

Book

Title: Methods in Molecular Biology

Pages: 137-146

ISBN: 978-1-4939-7035-3

Publisher: Humana Press

Indexing

Scopus - 3 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00T-3PC

DOI: 10.1007/978-1-4939-7037-7_8

Abstract (EN): In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10