Ozone therapy prevents the onset of dysplasia in HPV16-transgenic mice-A pre-clinical efficacy and safety analysis

• Type: Article in International Scientific Journal
• Date: 2018

Authors

C. Peirone (Author) Other; V. F. Mestre (Author) Other; B. Medeiros Fonseca (Author) Other; B. Colaço (Author) Other; M. J. Pires (Author) Other; T. Martins (Author) Other; R. M. Gil da Costa (Author) FEUP; M. J. Neuparth (Author) Other; R. Medeiros (Author) Other; Margarida M. S. M. Bastos (Author) FEUP; J. A. Marques-Magallanes (Author) Other; P. A. Oliveira (Author) Other

Journal

Title: Biomedicine & Pharmacotherapy

Vol. 104

Pages: 275-279

ISSN: 0753-3322

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 8 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00P-06N

DOI: 10.1016/j.biopha.2018.05.018

Abstract (EN): Infection with high-risk human papillomavirus (HPV), most often HPV16, is associated with the development of anogenital and oropharyngeal cancers. Recently, ozone therapy was reported to have considerable efficacy against rabbit VX2 tumors, induced by the cottontail rabbit papillomavirus. The present study aims to determine whether similar results can be obtained in HPV16-transgenic mice, possibly paving the way for new therapeutic options against HPV-induced cancers. HPV16-transgenic and wild-type, female, 20 weeks-old mice were injected intraperitoneally with medical O-3/O-2 (80 mL/kg, at O-3 50 mu g/mL), once a day, for 5 consecutive days. The animals were sacrificed at 25 weeks-old, and skin samples were analyzed histologically to study tumour progression. Blood and internal organ samples were used to study toxicological parameters. 85.7% of untreated transgenic mice showed dysplastic skin lesions, compared with 28.6% of O-3-treated mice. This was associated with a marked reduction of dermal inflammation associated with those lesions. No significant changes were observed in any toxicological parameters. These preliminary results support the hypothesis that O-3 therapy is effective against papillomavirus-induced lesions, particularly against those induced by the most common high-risk virus, HPV16. Further studies are needed to confirm the mechanisms underlying these effects.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5