Insights on sulfamethoxazole bio-transformation by environmental Proteobacteria isolates

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Patricia J M Reis (Author) FEUP; Vera Homem (Author) FEUP; Arminda Alves (Author) FEUP; Vitor Vilar (Author) FEUP; Célia Manaia (Author) Other; Olga C Nunes (Author) ICBAS

Journal

Title: Journal of Hazardous Materials

Vol. 358

Pages: 310-318

ISSN: 0304-3894

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 23 Citations

INSPEC

ScienceDirect (Elsevier)

Associated Projects

LSRE-LCM - Laboratório de Processos de Separação e Reação - Laboratório de Catálise e Materiais

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

Other information

Authenticus ID: P-00P-17B

DOI: 10.1016/j.jhazmat.2018.07.012

Abstract (EN): Although sulfonamide residues are frequently reported as freshwaters contaminants, information on the ability of native bacteria to modify these synthetic antibiotics is scarce. Our purpose was to investigate the potential of bacteria from different aquatic environments to cleave or transform sulfamethoxazole (SMX) and infer on their ability to reduce the toxicity of this antibiotic. From a collection of about 100 Proteobacteria, 47 strains previously isolated from drinking water, surface water, and wastewater grew in the presence of 200 mu M-SMX, and were further studied. Out of these, 14 strains, mostly from mineral drinking water, transformed SMX into equimolar amounts of the lesser toxic derivative N-4-acetyl-sulfamethoxazole. The highest percentage of SMX transformation was recorded for two strains affiliated to Pseudomonas mandelii. For P. mandelii McBPA4 higher SMX transformation rate and extent were observed in fed-batch (similar to 8 mu M-SMX/h, 81%) than in batch conditions (similar to 5 mu M-SMX/h, 25%), but similar specific transformation rates were found in both cultivation modes (similar to 20 mu mol(SMX)/g(cell dry weight)/h), indicating the dependence of the process on the microbial load. These results evidence that the capacity to transform synthetic antibiotics may be common among bacteria and highlight the potential of environmental bacteria in attenuating the potential adverse effects of pollution with sulfonamides.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9