Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Rui M. Gil da Costa (Author) FEUP; Rita Araújo (Author) Other; Joana M. O. Santos (Author) Other; Mara Fernandes (Author) Other; Tiago Neto (Author) Other; Hugo Sousa (Author) Other; Joana Ribeiro (Author) Other; Margarida M. S. M. Bastos (Author) FEUP; Paula A. Oliveira (Author) Other; Diogo Carmo (Author) Other; Fátima Casaca (Author) Other; Sandra Silva (Author) Other; Carlos Lopes (Author) Other; Rui Medeiros (Author) Other

Journal

Title: Anticancer Research

Vol. 37

Pages: 2913-2918

ISSN: 0250-7005

Publisher: International Institute of Anticancer Research

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00M-TZ1

DOI: 10.21873/anticanres.11644

Abstract (EN): Background/Aim: Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. Materials and Methods: Female transgenic (HPV16(+/-)) and wild-type (HPV16(-/-)) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR. Results: HPV16(+/-) mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16(+/-) animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice. Conclusion: In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6